Detailed information of TA system TA224254

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 914622..915457
• Replicon: chromosome
• Accession: NZ_CP086627
• Organism: Escherichia coli strain RIVM_C036569

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0A1A9A5
• Locus tag: LN356_RS04470
• Protein ID: WP_001564063.1
• Coordinates: 914622..914999 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0K5N986
• Locus tag: LN356_RS04475
• Protein ID: WP_021553056.1
• Coordinates: 915089..915457 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN356_RS04440 (909750)	909750..910898	-	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-
LN356_RS04445 (910970)	910970..911953	-	984	WP_001296394.1	KpsF/GutQ family sugar-phosphate isomerase	-
LN356_RS04450 (912763)	912763..912933	-	171	Protein_875	IS110 family transposase	-
LN356_RS04455 (913276)	913276..914118	-	843	Protein_876	DUF4942 domain-containing protein	-
LN356_RS04460 (914203)	914203..914397	-	195	WP_024181941.1	DUF957 domain-containing protein	-
LN356_RS04465 (914476)	914476..914625	-	150	Protein_878	DUF5983 family protein	-
LN356_RS04470 (914622)	914622..914999	-	378	WP_001564063.1	TA system toxin CbtA family protein	Toxin
LN356_RS04475 (915089)	915089..915457	-	369	WP_021553056.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LN356_RS04480 (915620)	915620..915841	-	222	WP_000692345.1	DUF987 domain-containing protein	-
LN356_RS04485 (915906)	915906..916382	-	477	WP_021553055.1	RadC family protein	-
LN356_RS04490 (916398)	916398..916877	-	480	WP_001564060.1	antirestriction protein	-
LN356_RS04495 (917143)	917143..917961	-	819	WP_001564059.1	DUF932 domain-containing protein	-
LN356_RS04500 (918051)	918051..918284	-	234	WP_001278293.1	DUF905 family protein	-
LN356_RS04505 (918290)	918290..918967	-	678	WP_001564058.1	hypothetical protein	-
LN356_RS04510 (919118)	919118..919798	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX	797594..992637	195043
	-	flank	IS/Tn	-	-	912763..912918	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14094.09 Da
• Isoelectric Point: 7.8045

>T224254 WP_001564063.1 NZ_CP086627:c914999-914622 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T224254 NZ_CP119862:c2881144-2881041 [Citrobacter sp. S171]
GGCAGGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13615.39 Da
• Isoelectric Point: 7.0264

>AT224254 WP_021553056.1 NZ_CP086627:c915457-915089 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT224254 NZ_CP119862:2881035-2881246 [Citrobacter sp. S171]
ACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCCTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTCTGTGGCTAAAGTGG
CCGGAAAAAAAGGATGCTTGTCACCCGCTACAACGTAAAAACCGCAAGTTCT

Structures

Toxin

• AlphaFold DB: A0A0A1A9A5

Antitoxin

• AlphaFold DB: A0A0K5N986