Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4731666..4732163 Replicon chromosome
Accession NZ_CP086626
Organism Escherichia coli strain RIVM_C037134

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LN362_RS23095 Protein ID WP_001173343.1
Coordinates 4731666..4731839 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LN362_RS23100 Protein ID WP_000398619.1
Coordinates 4731867..4732163 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN362_RS23085 (4728336) 4728336..4729793 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
LN362_RS23090 (4730030) 4730030..4731547 + 1518 WP_001550472.1 lysine--tRNA ligase -
LN362_RS23095 (4731666) 4731666..4731839 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LN362_RS23100 (4731867) 4731867..4732163 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LN362_RS23105 (4732392) 4732392..4732664 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LN362_RS23110 (4732676) 4732676..4732906 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
LN362_RS23115 (4733086) 4733086..4734717 + 1632 WP_001216477.1 sensor histidine kinase -
LN362_RS23120 (4734714) 4734714..4735433 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T224249 WP_001173343.1 NZ_CP086626:c4731839-4731666 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T224249 NZ_CP119862:39579-39920 [Citrobacter sp. S171]
ATGTGGGATGTTGAAACAACGGACGCTTTCGACAGGTGGTTTGACGAACAGACTGAAGCACTCAAGGAAGACATGCTCGC
TGCTATGATGATTTTGTCCGAATATGGCCCCCAATTGGGAAGACCGTTTGCGGATACGGTGAACGACTCTGCTTTTTCGA
ATATGAAAGAGTTACGCGTTCAGCATCAAGGTAGCCCAATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGTCATGGCATC
GTGTTGTGCGCTGGAGATAAAACGGGGTTAAACGAGAAGAAATTCTATAAGGATATGATTAAGTTTGCAGATGCTGAGTA
CAGAAAACATCTTAATAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT224249 WP_000398619.1 NZ_CP086626:c4732163-4731867 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT224249 NZ_CP119862:39930-40229 [Citrobacter sp. S171]
ATGGCTACCCTCAAAGAGTTGATGGCTAAACAAACCCCGGAAAGTCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCA
CCAGGCTATTGCGTTAAATATGTTAAGGGAAGAACTTAACATGTCCCAGGCAGAACTGGCCGCGGCAATGGGCGTTAAGC
AGCCAACCATTGCAAAAATGGAACAGGCTGAAAATGATCCTCGTCTCTCTACGTTGAAACGTTACGTAGCAGCGCTGGGT
GGCGAGTTAAGTATTGATGTTAAGTTGCCCACCGGTAAGAGAGTTGCGTTCCATATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References