Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4480523..4481099 Replicon chromosome
Accession NZ_CP086626
Organism Escherichia coli strain RIVM_C037134

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LN362_RS21815 Protein ID WP_001297643.1
Coordinates 4480812..4481099 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag LN362_RS21810 Protein ID WP_000063160.1
Coordinates 4480523..4480825 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN362_RS21795 (4477119) 4477119..4479269 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
LN362_RS21800 (4479319) 4479319..4479522 + 204 WP_000467859.1 YbdD/YjiX family protein -
LN362_RS21805 (4479533) 4479533..4480489 + 957 WP_001297640.1 GTPase -
LN362_RS21810 (4480523) 4480523..4480825 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
LN362_RS21815 (4480812) 4480812..4481099 - 288 WP_001297643.1 BrnT family toxin Toxin
LN362_RS21825 (4482310) 4482310..4483542 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
LN362_RS21830 (4483583) 4483583..4484863 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4481298..4481591 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T224247 WP_001297643.1 NZ_CP086626:c4481099-4480812 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T224247 NZ_CP119773:c4039665-4039585 [Dickeya fangzhongdai]
AATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGGGCACAGGAATCGTGTTCGGTCTTTTTT
T

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT224247 WP_000063160.1 NZ_CP086626:c4480825-4480523 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT224247 NZ_CP119773:4039583-4039665 [Dickeya fangzhongdai]
ATAAAAAAAGACCGAACACGATTCCTGTGCCCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References