Detailed information of TA system TA224245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4127207..4128896
• Replicon: chromosome
• Accession: NZ_CP086626
• Organism: Escherichia coli strain RIVM_C037134

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: LN362_RS20150
• Protein ID: WP_000365809.1
• Coordinates: 4127207..4128127 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LN362_RS20155
• Protein ID: WP_001550661.1
• Coordinates: 4128114..4128896 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN362_RS20135 (4123297)	4123297..4124424	-	1128	WP_001550663.1	MFS transporter	-
LN362_RS20140 (4124566)	4124566..4125624	-	1059	WP_122987197.1	alpha/beta hydrolase	-
LN362_RS20145 (4125889)	4125889..4126773	+	885	WP_001119037.1	LysR family transcriptional regulator	-
LN362_RS20150 (4127207)	4127207..4128127	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LN362_RS20155 (4128114)	4128114..4128896	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
LN362_RS20160 (4129212)	4129212..4132421	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
LN362_RS20165 (4132768)	4132768..4133238	+	471	WP_000201185.1	hypothetical protein	-
LN362_RS20170 (4133235)	4133235..4133337	+	103	Protein_3957	helix-turn-helix domain-containing protein	-
LN362_RS20175 (4133429)	4133429..4133587	+	159	Protein_3958	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T224245 WP_000365809.1 NZ_CP086626:c4128127-4127207 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T224245 NZ_CP119773:3308306-3308620 [Dickeya fangzhongdai]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCACCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
ACAATTGAATCGTCGGATAGTGATCCCATTGCTCCCTATTGAAAAGTATCCGGCAGGCCGCCGACCGGATCGCCTTGTCC
CCGTCGTCATGCTGACGGACGGCAAAGAATACGCCGTAATGACGCACGAGCTGGCAAGTATCCCTGTGCAGGTGTTGGGT
GCGGTGTTCTGTGATGCGTCGCAGTACCGTAATCAGGTAAAAGCCGCAATAGATTTCCTCATTGACGGCATTTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT224245 WP_001550661.1 NZ_CP086626:c4128896-4128114 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT224245 NZ_CP119773:3308067-3308303 [Dickeya fangzhongdai]
ATGACCGCGAAACAACGTAACACGCAGAGCGTGACCATGACGGTAGAGCGTGCCTTACTGGTCCGGGCGCGTGAAGCGGG
GATTAACCTGAGTGCCACCCTGACAACCGCTCTGGATGCAGAGCTTCGCCACCATGAAGCGAAAAAATGGCAGGAAGAGA
ACAGGGAAGCCATCGACGCATTAAACCGTTTTCATGATGAGCACGGCTGTTTCAGCGATGAATACAGGACGTTTTGA

Structures

Toxin

• AlphaFold DB: V0Z9M0