Detailed information of TA system TA224241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2837454..2838092
• Replicon: chromosome
• Accession: NZ_CP086626
• Organism: Escherichia coli strain RIVM_C037134

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: LN362_RS13915
• Protein ID: WP_000813795.1
• Coordinates: 2837916..2838092 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LN362_RS13910
• Protein ID: WP_076797675.1
• Coordinates: 2837454..2837870 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN362_RS13890 (2832606)	2832606..2833547	-	942	WP_001251315.1	ABC transporter permease	-
LN362_RS13895 (2833548)	2833548..2834561	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
LN362_RS13900 (2834579)	2834579..2835724	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
LN362_RS13905 (2835969)	2835969..2837375	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
LN362_RS13910 (2837454)	2837454..2837870	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LN362_RS13915 (2837916)	2837916..2838092	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LN362_RS13920 (2838314)	2838314..2838544	+	231	WP_023910283.1	YncJ family protein	-
LN362_RS13925 (2838636)	2838636..2840597	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LN362_RS13930 (2840670)	2840670..2841206	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
LN362_RS13935 (2841298)	2841298..2842470	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T224241 WP_000813795.1 NZ_CP086626:c2838092-2837916 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T224241 NZ_CP119773:2306280-2306711 [Dickeya fangzhongdai]
GTGGCCCAGTGGCAATGTGATTTGCGTGTATCCTGGCGCATGCAGTTGTTTTCTCTGCTGACGCACGGGTTCCTGGTGCT
GATGATCCTGCTGGCTCCCTGGCCGGACGGCTATGCGCCGCTGTGGCTGGGGTTGGTGACGCTGGTGGTGTTCGGTTTCG
TGCGCAGTCAGCGCATCATCAAGTCGCGTCAGGGGGAAATTTCCCTGCATGGGGAAAACCAACTGCACTGGCAACAGCGT
GACTGGCAGATAGCCCGGCGACCGTGGGTGATGCGCAACGGCGTGTTGCTGTCGTTGCGGGCGACGACCGGCAAAGGGCA
TCAGCGCCTGTGGCTGGCGTCAGACAGTATGGGCAATGAAGAGTGGCGGCTGTTGCGCCAACTGTTGCTGCAGCATCCGC
TGCAGGGCACGGAATCCTCCCGCCATCATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT224241 WP_076797675.1 NZ_CP086626:c2837870-2837454 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT224241 NZ_CP119773:2306033-2306299 [Dickeya fangzhongdai]
ATGGACATCAATAACAAATCACGCATTCACTGGGCGTGCAGACGCGGAATGCGTGAACTGGATATTTCCATCATGCCGTT
TTTCGAGCATGAGTACGACACGCTGAGCGACGACGACAAGCAGCATTTCATCCGTCTGTTGCAGTGCGACGATCCCGACT
TGTTCAACTGGCTGATGAACCACGGCGAGCCGGTGGATCCGGAGCTGAAGCGCATGGTTTCCCTTATTCAGACGCGAAAT
AAAGACCGTGGCCCAGTGGCAATGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1