Detailed information of TA system TA224240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2732740..2734328
• Replicon: chromosome
• Accession: NZ_CP086626
• Organism: Escherichia coli strain RIVM_C037134

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A829LC11
• Locus tag: LN362_RS13470
• Protein ID: WP_001551128.1
• Coordinates: 2733006..2734328 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LN362_RS13465
• Protein ID: WP_001296726.1
• Coordinates: 2732740..2733006 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN362_RS13465 (2732740)	2732740..2733006	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LN362_RS13470 (2733006)	2733006..2734328	+	1323	WP_001551128.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
LN362_RS13475 (2734528)	2734528..2735880	-	1353	WP_001551127.1	SIR2 family protein	-
LN362_RS13480 (2736095)	2736095..2737127	-	1033	Protein_2646	ISNCY family transposase	-
LN362_RS13485 (2737494)	2737494..2738393	-	900	WP_001551125.1	LysR family transcriptional regulator	-
LN362_RS13490 (2738532)	2738532..2738840	+	309	WP_001221046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2736095..2736913	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49270.88 Da
• Isoelectric Point: 8.2472

>T224240 WP_001551128.1 NZ_CP086626:2733006-2734328 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T224240 NZ_CP119773:c2176729-2176424 [Dickeya fangzhongdai]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCCAAGCGGCGTATGGTCATTCCGATTATCGAAGCACATCATCTCTCTGAGAAAGTGAATAAAACGCTGTTCCCGCTGA
TCCGCATCGACAGCGAAGACTATCGGCTGATGACGACGGAGCTATCGAGCGTTTCCGTTGAAGTCATTGGTGAAGTGACT
GCGGATCTTGGCGACTACGCGGATGAGATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT224240 WP_001296726.1 NZ_CP086626:2732740-2733006 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT224240 NZ_CP119773:c2176950-2176732 [Dickeya fangzhongdai]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCCGCCGGGGTCAATATTTCCGGGCT
GGTGAATGAGGCCATTGGCAAAGAAGCCCGCCGCATCAGGGCTGAAGAGTGGAAGAAGGAGAATCGCGAGGGGATGGAAG
AAGTCGCCCGATTTATCGCACAGCATGGCTCCTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829LC11