Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4508969..4509545 Replicon chromosome
Accession NZ_CP086625
Organism Escherichia coli strain RIVM_C037166

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LN360_RS22030 Protein ID WP_001297643.1
Coordinates 4509258..4509545 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag LN360_RS22025 Protein ID WP_000063160.1
Coordinates 4508969..4509271 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN360_RS22010 (4505565) 4505565..4507715 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
LN360_RS22015 (4507765) 4507765..4507968 + 204 WP_000467859.1 YbdD/YjiX family protein -
LN360_RS22020 (4507979) 4507979..4508935 + 957 WP_001297640.1 GTPase -
LN360_RS22025 (4508969) 4508969..4509271 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
LN360_RS22030 (4509258) 4509258..4509545 - 288 WP_001297643.1 BrnT family toxin Toxin
LN360_RS22040 (4510756) 4510756..4511988 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
LN360_RS22045 (4512029) 4512029..4513309 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4509744..4510037 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T224220 WP_001297643.1 NZ_CP086625:c4509545-4509258 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T224220 NZ_CP119754:362199-362567 [Superficieibacter sp. HKU1]
ATGACACTGCAACTTATCTCAGCGGAAGAGATTGTATCGTTTCATGACAAACTGCTGAGCGTGACGCCCGGTGTACCCGG
TATGCCCGATCCAGGGCGCGCTGAAGCGTTGCTCTATCGTGTTCTCAATAAATATGATTATGAAGGGATTTCTGGCCTGT
GGAATCTGGCGGCCATGTATCTGCTGGCTATTTCACGTGGTCATATATTCAATGATGGTAATAAGCGTACGGCGTTGTTT
ATTGCGTTGCTTTTTCTGAAGCGTAATGGCGTGTCACTTCCTGCTAATCCGGCTTACGTTGAGTTAACCGTCGACGCTGC
TGCGGGGCGTTTATCTCTTGAGGAAATTGCCGGGCGCTTGCGCGGGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT224220 WP_000063160.1 NZ_CP086625:c4509271-4508969 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT224220 NZ_CP119754:361981-362202 [Superficieibacter sp. HKU1]
ATGCGTACAATTAACTATAGCGAAGCCCGGCAAAATCTTGCGGCCGCCCTTGAAACGGCTGTGACAGGTACGCCAGTGAC
CATTACACGACGTGGTCGCAAACCCGCCGTCATTATTAGCGCCGAAGAGTTTGAACGCTATCAAACGGCAAAACTTGATG
CAGAGTTTGCTGCCATCATGATGGTACATGGAAATGAAATAAGGGAACTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References