Detailed information of TA system TA224218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4155653..4157342
• Replicon: chromosome
• Accession: NZ_CP086625
• Organism: Escherichia coli strain RIVM_C037166

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: LN360_RS20365
• Protein ID: WP_000365809.1
• Coordinates: 4155653..4156573 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LN360_RS20370
• Protein ID: WP_001550661.1
• Coordinates: 4156560..4157342 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN360_RS20350 (4151743)	4151743..4152870	-	1128	WP_001550663.1	MFS transporter	-
LN360_RS20355 (4153012)	4153012..4154070	-	1059	WP_122987197.1	alpha/beta hydrolase	-
LN360_RS20360 (4154335)	4154335..4155219	+	885	WP_001119037.1	LysR family transcriptional regulator	-
LN360_RS20365 (4155653)	4155653..4156573	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LN360_RS20370 (4156560)	4156560..4157342	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
LN360_RS20375 (4157658)	4157658..4160867	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
LN360_RS20380 (4161214)	4161214..4161684	+	471	WP_000201185.1	hypothetical protein	-
LN360_RS20385 (4161681)	4161681..4161783	+	103	Protein_3997	helix-turn-helix domain-containing protein	-
LN360_RS20390 (4161875)	4161875..4162033	+	159	Protein_3998	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T224218 WP_000365809.1 NZ_CP086625:c4156573-4155653 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T224218 NZ_CP119754:324403-324879 [Superficieibacter sp. HKU1]
ATGGAAGTAACCACGCCCAAAATACTCACTGACACCCATGTGATGACTGATTTTTGTTGTGGAAATAGCGTCCTGGATGA
CTGGTTAATGCGGCGAGCATTAAAAAAACAGGCGTTAAACGCTTCCCGAACGTTTGTTACCTGTGAGAGCGGGACCCGGA
ATGTGGTTGGCTATTACTCACTGGCTTCGGGTTCAGTTACACATCATGCCGTCCCGCGTGGATTGCGGCAGAATATGCCC
GAACCCATCCCGGTTATTCTCCTGGGTCGTCTGGCTGTCGATCAACGTTATCAGGGCCAGGATATGGGTAAGTGGCTCCT
TAATGATGTTGTTAACCGCGTCATTAATGTCGCGGAGCAAATTGGGGTAAAAGCCATGATGGTTCACGCCATCAACGAAG
ACGCCCGGAGATTCTATAAACATTTCGGCTTCGTGCAGTCGGTTATCGCAGAAGATACCCTGTTCTGGCGTTTATAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT224218 WP_001550661.1 NZ_CP086625:c4157342-4156560 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT224218 NZ_CP119754:324116-324412 [Superficieibacter sp. HKU1]
ATGCCAGCATCGACAGCAAAAGTTTCGAAAAAATTCAACCGGGATAACCAAATTAACCTGCGCGCCAGCGATGCTGAACG
CGCCATCATTGATTATGCTGCCAGCTTATTGAATAAATCACGTACAGATTTCATTCTTGAACTTGCTTACAACGAAGCAC
AAAATATTATTTTAGATCAACGGCTTTTTGTCCTGGATGCGGATAAATATCAGGCGTTTGTCGAGCAGCTTGAAGCGCCT
CTGGAGAATGTCGAAGGTCGTAATCGTTTAATGGATGTGAAACCTGAATGGAAGTAA

Structures

Toxin

• AlphaFold DB: V0Z9M0