Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3950984..3951602 Replicon chromosome
Accession NZ_CP086625
Organism Escherichia coli strain RIVM_C037166

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LN360_RS19405 Protein ID WP_001291435.1
Coordinates 3951384..3951602 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LN360_RS19400 Protein ID WP_000344800.1
Coordinates 3950984..3951358 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN360_RS19390 (3946073) 3946073..3947266 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LN360_RS19395 (3947289) 3947289..3950438 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LN360_RS19400 (3950984) 3950984..3951358 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LN360_RS19405 (3951384) 3951384..3951602 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LN360_RS19410 (3951774) 3951774..3952325 + 552 WP_000102564.1 maltose O-acetyltransferase -
LN360_RS19415 (3952441) 3952441..3952911 + 471 WP_000136192.1 YlaC family protein -
LN360_RS19420 (3953075) 3953075..3954625 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LN360_RS19425 (3954667) 3954667..3955020 - 354 WP_085334692.1 DUF1428 family protein -
LN360_RS19435 (3955399) 3955399..3955710 + 312 WP_000409911.1 MGMT family protein -
LN360_RS19440 (3955741) 3955741..3956313 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224217 WP_001291435.1 NZ_CP086625:3951384-3951602 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T224217 NZ_CP119754:c124527-124186 [Superficieibacter sp. HKU1]
ATGGACGCGCTTTTTATTGAACTACCGCCGTTTGAACGGCACCGCGCGGAGTACTTCAGCGATGAAGAATTCCGTGCCTT
CCAGCAATTTTTGCTGAAAAACCCCACGTGCGGGGATGTGATCCCGGATGCTGGTGGGTTACGTAAGGTTCGTTTTTTAG
ATTCGCGCAGGAATAAGGGAAAGCGTGGGGGCATCCGGGTTATTTACTACTGGTACCTCGAAAAATCGCACTTTTTACTG
TTCACTTTGTACGATAAAGATCAGCTTGATGATCTCACTACTCAGCAACGCAACGTCCTGCGCCAGCTCCTGGAACAGGC
AAAACAGAGGGGCAAATCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT224217 WP_000344800.1 NZ_CP086625:3950984-3951358 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT224217 NZ_CP119754:c124189-123878 [Superficieibacter sp. HKU1]
ATGACTCAACGAAATATTTTTAGCGAGCTGACAGAAGGATTTCAGGCATGGGAAGAGTATGACGCAGGGAAAATCACGCT
GCGTACTCACCGCGTTAATACTGAAACCCCGGCGATCTCTCCTGATGATTTGGTCAAAGTCCGCGAAAAATTAAATCTCT
CACAGGCTGTTTTTGCACAGTACCTTCATGCTGGCCTCAGAACATATCAGAACTGGGAACAGGGACTGGCCCGACCCAAT
AAACAGGCCGTGTTGCTGATCCGTATGATTGAGAAAAGCCCGTCAGTATTGTCCCAACTGGCAATTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References