Detailed information of TA system TA224216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3497936..3498641
• Replicon: chromosome
• Accession: NZ_CP086625
• Organism: Escherichia coli strain RIVM_C037166

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LN360_RS17290
• Protein ID: WP_000539521.1
• Coordinates: 3497936..3498322 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LN360_RS17295
• Protein ID: WP_001280945.1
• Coordinates: 3498312..3498641 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN360_RS17270 (3493940)	3493940..3494566	+	627	WP_001295292.1	glutathione S-transferase GstB	-
LN360_RS17275 (3494563)	3494563..3495678	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
LN360_RS17280 (3495789)	3495789..3496172	-	384	WP_000497137.1	biofilm formation regulator BssR	-
LN360_RS17285 (3496385)	3496385..3497710	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LN360_RS17290 (3497936)	3497936..3498322	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LN360_RS17295 (3498312)	3498312..3498641	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LN360_RS17300 (3498711)	3498711..3500039	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
LN360_RS17305 (3500047)	3500047..3502395	-	2349	WP_001550886.1	EAL domain-containing protein	-
LN360_RS17310 (3502573)	3502573..3503484	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T224216 WP_000539521.1 NZ_CP086625:3497936-3498322 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T224216 NZ_CP119754:c82025-81705 [Superficieibacter sp. HKU1]
ATGCATATTTCGACTGTACCGAAAACAGTGACGGTTTCGTCACGCCTGTCTCCTGTCCAAGTTTGGCAAAAACTATTAAC
GTATATTTTAGAGCATCATTACGGCCTCACGATCAACGACACACCGTTCAGTAACGATACGACGATTCAGGAACATATAG
AGGCGGGAGTGAATCTTACTGATGCTGTTAATTTCCTTGTGGAACGATTCGAGCTTGTGCGCATTGACCAGAAGAGTTTC
TCCTGGCAGGATCAAGAACCCTGGATAACATCTTTGGATGTTCATCGGGCACAGTCCAACCTTGGCTTGAAACGCCCATA
A

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT224216 WP_001280945.1 NZ_CP086625:3498312-3498641 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT224216 NZ_CP119754:c82423-82067 [Superficieibacter sp. HKU1]
ATGCAGTCGACCACCATATCCGGCACATCGGCGGAGAACACATCCTGCCTTCAGTGGGGATTAAAACGTAATATCACGCC
GTGCTTCGGCGCTCGTCTGGTACAGGAAGGCAACCGCATATACTTTTTGGCTGACCGGGCAGGATTTAATGGCTCATTCA
GTGACGCAGAAGCATTGAGTCTGGACCAAGCGTTTCCACTAATGGTGAAACAGCTTGAGCGGATGCTGACAACTGGCGAG
CTTGATCCTCACAACCAGCACTGCGTCACACTGCATCACAATGGACTTACTTGTGAAGCTGACACTCTCGGCTCTTATGG
CTACGTTTATATCGCCATTTATCCCCATCAGCTATAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6