Detailed information of TA system TA224215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3207913..3208697
• Replicon: chromosome
• Accession: NZ_CP086625
• Organism: Escherichia coli strain RIVM_C037166

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: LN360_RS15835
• Protein ID: WP_000613626.1
• Coordinates: 3208203..3208697 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: LN360_RS15830
• Protein ID: WP_001110447.1
• Coordinates: 3207913..3208206 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN360_RS15820 (3203063)	3203063..3204022	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
LN360_RS15825 (3204595)	3204595..3207780	+	3186	WP_001550951.1	ribonuclease E	-
LN360_RS15830 (3207913)	3207913..3208206	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
LN360_RS15835 (3208203)	3208203..3208697	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
LN360_RS15840 (3208792)	3208792..3209745	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
LN360_RS15845 (3209757)	3209757..3211400	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
LN360_RS15850 (3211466)	3211466..3212407	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
LN360_RS15855 (3212407)	3212407..3213504	-	1098	WP_001441925.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T224215 WP_000613626.1 NZ_CP086625:3208203-3208697 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T224215 NZ_CP119753:c1145178-1144789 [Microbacter sp. GSS18]
GTGGCTGATACGTCCGCCCTCGTGGCCGTCGTCTTCGGCGAGGCCGACGCGCGCGCCTTCGCCTCGGTGCTCGCAGCCCA
CGCCGGTGACGTCAGCGTGAGCGCCGCCACCCTGGTCGAGGCGAGGATCGTCGTGGAGGGTCGGCAGGGCACGGAAGCGG
CTGCCGACCTGGATCGGCTGCTCACGCGACTCGGCGCGCACGTGGTGGCGGTGGACGACGCGCAGGCTTCTCTCGCGACG
TCGGCCTGGCGGCGCTTCGGGAAGGGCCGGCACGACGCGGGGCTGAACTTCGGTGACTGCTTCTCGTACGCGCTGTCCAA
GCACCTCGACGTCCCGCTGCTGTACAAGGGCGACGACTTCTCGCGAACCGACGTCGCGTCGGCGATGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT224215 WP_001110447.1 NZ_CP086625:3207913-3208206 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT224215 NZ_CP119753:c1145441-1145187 [Microbacter sp. GSS18]
ATGGCCCTCAACATCAAGGATCCGGAGACCGACCGGATCGCGCGCGAGCTTGCGGCTGCCACGGGGGAGAGCATCACCGT
GGCGGCGCGCATCGCGATGGAGGAGCGGCTGCGCCGCGTGCGTGCGAGCGGCGCGGAGCAGGCTGTTCGCGATGAGCTCG
ATGCGATCATCGCGCGTGGGCGCAGCCGACGCAGTCTCGATGACCGCGGCGCGGATGAGATCGTCGGCTACGACGAGGAT
GGACTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6