Detailed information of TA system TA224214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2829937..2830575
• Replicon: chromosome
• Accession: NZ_CP086625
• Organism: Escherichia coli strain RIVM_C037166

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: LN360_RS13875
• Protein ID: WP_000813795.1
• Coordinates: 2830399..2830575 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LN360_RS13870
• Protein ID: WP_076797675.1
• Coordinates: 2829937..2830353 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN360_RS13850 (2825089)	2825089..2826030	-	942	WP_001251315.1	ABC transporter permease	-
LN360_RS13855 (2826031)	2826031..2827044	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
LN360_RS13860 (2827062)	2827062..2828207	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
LN360_RS13865 (2828452)	2828452..2829858	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
LN360_RS13870 (2829937)	2829937..2830353	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LN360_RS13875 (2830399)	2830399..2830575	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LN360_RS13880 (2830797)	2830797..2831027	+	231	WP_023910283.1	YncJ family protein	-
LN360_RS13885 (2831119)	2831119..2833080	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LN360_RS13890 (2833153)	2833153..2833689	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
LN360_RS13895 (2833781)	2833781..2834953	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T224214 WP_000813795.1 NZ_CP086625:c2830575-2830399 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T224214 NZ_CP119753:c135998-135630 [Microbacter sp. GSS18]
GTGAGCGATGCGCTCCCGCTCCTCCGACGCGGACACATCGTGCTGGTGAATCTCGACCCCGCGGTCGGCTCTGAGGCAGC
GAAGACCCGCCCCGCCATCGTCGTGAGCAACAACACCGCGAACGCCAGTGCGCTCCGCAGCGACCGCGGCGTCATCACCG
TGGTTCCCCTCACCTCGAACGTCGCACGGGTGCATGGGTTCCAGGTGCTCGTGGATCCCGAGAGCTCCGGGCTCACCCGC
ACATCCAAGGCGCAGGCCGAGCGGGTCCGGACGGTCTCGATCGCACGCGTGAGCGCCGTCACGGGGTGGCTGGGTGCCGA
GCAGATGGGCGCCGTCGACGCCGCGCTGCGCCTCCATCTGTCGCTCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT224214 WP_076797675.1 NZ_CP086625:c2830353-2829937 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT224214 NZ_CP119753:c136216-135995 [Microbacter sp. GSS18]
ATGTCGTACGCCAAGGTCAGTCTGAGCCTCAGCGATGCCGACGTCGCCTTCCTCGACTCCGAGACCCTGTCGGGCGCCTA
CCCGAGCCGATCGGCGGCCGTGCAGGACGCCGCGCGGATGCTGCGCGAGAGCCGCCTCGCGGATGCCTACGCCCAGGCCT
TCGAGGAGTGGGACGACGACGCGTGGGATGCCACATCGGGCGACGGGATCTCAGCCGCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1