Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1084183..1084837 Replicon chromosome
Accession NZ_CP086625
Organism Escherichia coli strain RIVM_C037166

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LN360_RS05310 Protein ID WP_000244781.1
Coordinates 1084430..1084837 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0V9GG32
Locus tag LN360_RS05305 Protein ID WP_001564007.1
Coordinates 1084183..1084449 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN360_RS05285 (1080271) 1080271..1081704 - 1434 WP_001564009.1 6-phospho-beta-glucosidase BglA -
LN360_RS05290 (1081749) 1081749..1082060 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
LN360_RS05295 (1082224) 1082224..1082883 + 660 WP_000250274.1 hemolysin III family protein -
LN360_RS05300 (1082960) 1082960..1083940 - 981 WP_001564008.1 tRNA-modifying protein YgfZ -
LN360_RS05305 (1084183) 1084183..1084449 + 267 WP_001564007.1 FAD assembly factor SdhE Antitoxin
LN360_RS05310 (1084430) 1084430..1084837 + 408 WP_000244781.1 protein YgfX Toxin
LN360_RS05315 (1084877) 1084877..1085398 - 522 WP_001055874.1 flavodoxin FldB -
LN360_RS05320 (1085510) 1085510..1086406 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LN360_RS05325 (1086431) 1086431..1087141 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LN360_RS05330 (1087147) 1087147..1088880 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T224202 WP_000244781.1 NZ_CP086625:1084430-1084837 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T224202 NZ_CP119746:3614157-3614375 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10565.15 Da        Isoelectric Point: 5.1599

>AT224202 WP_001564007.1 NZ_CP086625:1084183-1084449 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWMMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT224202 NZ_CP119746:3613757-3614131 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9GG32

References