Detailed information of TA system TA224200

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 882964..883799
• Replicon: chromosome
• Accession: NZ_CP086625
• Organism: Escherichia coli strain RIVM_C037166

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0A1A9A5
• Locus tag: LN360_RS04245
• Protein ID: WP_001564063.1
• Coordinates: 882964..883341 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0K5N986
• Locus tag: LN360_RS04250
• Protein ID: WP_021553056.1
• Coordinates: 883431..883799 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN360_RS04215 (878092)	878092..879240	-	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-
LN360_RS04220 (879312)	879312..880295	-	984	WP_001296394.1	KpsF/GutQ family sugar-phosphate isomerase	-
LN360_RS04225 (881105)	881105..881275	-	171	Protein_829	IS110 family transposase	-
LN360_RS04230 (881618)	881618..882460	-	843	Protein_830	DUF4942 domain-containing protein	-
LN360_RS04235 (882545)	882545..882739	-	195	WP_024181941.1	DUF957 domain-containing protein	-
LN360_RS04240 (882818)	882818..882967	-	150	Protein_832	DUF5983 family protein	-
LN360_RS04245 (882964)	882964..883341	-	378	WP_001564063.1	TA system toxin CbtA family protein	Toxin
LN360_RS04250 (883431)	883431..883799	-	369	WP_021553056.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LN360_RS04255 (883962)	883962..884183	-	222	WP_000692345.1	DUF987 domain-containing protein	-
LN360_RS04260 (884248)	884248..884724	-	477	WP_021553055.1	RadC family protein	-
LN360_RS04265 (884740)	884740..885219	-	480	WP_001564060.1	antirestriction protein	-
LN360_RS04270 (885485)	885485..886303	-	819	WP_001564059.1	DUF932 domain-containing protein	-
LN360_RS04275 (886393)	886393..886626	-	234	WP_001278293.1	DUF905 family protein	-
LN360_RS04280 (886632)	886632..887309	-	678	WP_001564058.1	hypothetical protein	-
LN360_RS04285 (887460)	887460..888140	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX	765937..960966	195029
	-	flank	IS/Tn	-	-	881105..881260	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14094.09 Da
• Isoelectric Point: 7.8045

>T224200 WP_001564063.1 NZ_CP086625:c883341-882964 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T224200 NZ_CP119746:2830583-2830690 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13615.39 Da
• Isoelectric Point: 7.0264

>AT224200 WP_021553056.1 NZ_CP086625:c883799-883431 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT224200 NZ_CP119746:c2830530-2830469 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A0A1A9A5

Antitoxin

• AlphaFold DB: A0A0K5N986