Detailed information of TA system TA022420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1529716..1530354
• Replicon: chromosome
• Accession: NC_009800
• Organism: Escherichia coli HS

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECHS_RS07525
• Protein ID: WP_000813794.1
• Coordinates: 1529716..1529892 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECHS_RS07530
• Protein ID: WP_001270286.1
• Coordinates: 1529938..1530354 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECHS_RS07505 (EcHS_A1518)	1525335..1526549	-	1215	WP_071607224.1	BenE family transporter YdcO	-
ECHS_RS07510 (EcHS_A1517)	1526602..1527138	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECHS_RS07515 (EcHS_A1519)	1527211..1529172	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECHS_RS07520 (EcHS_A1520)	1529264..1529494	-	231	WP_000494244.1	YncJ family protein	-
ECHS_RS07525	1529716..1529892	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECHS_RS07530 (EcHS_A1522)	1529938..1530354	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECHS_RS07535 (EcHS_A1523)	1530433..1531839	+	1407	WP_000760619.1	PLP-dependent aminotransferase family protein	-
ECHS_RS07540 (EcHS_A1524)	1532084..1533229	+	1146	WP_000047421.1	ABC transporter substrate-binding protein	-
ECHS_RS07545 (EcHS_A1525)	1533247..1534260	+	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
ECHS_RS07550 (EcHS_A1526)	1534261..1535202	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T22420 WP_000813794.1 NC_009800:1529716-1529892 [Escherichia coli HS]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T22420 NC_009800:1529716-1529892 [Escherichia coli HS]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT22420 WP_001270286.1 NC_009800:1529938-1530354 [Escherichia coli HS]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT22420 NC_009800:1529938-1530354 [Escherichia coli HS]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB