Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4646302..4646897 Replicon chromosome
Accession NZ_CP086624
Organism Escherichia coli strain RIVM_C037791

Toxin (Protein)


Gene name chpB Uniprot ID A0A0V9NWK6
Locus tag LN354_RS22680 Protein ID WP_019842229.1
Coordinates 4646302..4646652 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID L4JJX7
Locus tag LN354_RS22685 Protein ID WP_001223213.1
Coordinates 4646646..4646897 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN354_RS22660 (4641632) 4641632..4642654 - 1023 WP_001550507.1 ABC transporter permease -
LN354_RS22665 (4642668) 4642668..4644170 - 1503 WP_001550506.1 sugar ABC transporter ATP-binding protein -
LN354_RS22670 (4644302) 4644302..4645258 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
LN354_RS22675 (4645568) 4645568..4646098 + 531 WP_000055075.1 inorganic diphosphatase -
LN354_RS22680 (4646302) 4646302..4646652 - 351 WP_019842229.1 endoribonuclease toxin ChpB Toxin
LN354_RS22685 (4646646) 4646646..4646897 - 252 WP_001223213.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
LN354_RS22690 (4647109) 4647109..4647450 - 342 WP_001550504.1 gamma-glutamylcyclotransferase -
LN354_RS22695 (4647453) 4647453..4651232 - 3780 WP_001550503.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-115)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12483.44 Da        Isoelectric Point: 5.6097

>T224194 WP_019842229.1 NZ_CP086624:c4646652-4646302 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVWMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Download         Length: 351 bp

>T224194 NZ_CP119746:1923263-1923514 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9331.64 Da        Isoelectric Point: 4.6509

>AT224194 WP_001223213.1 NZ_CP086624:c4646897-4646646 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT224194 NZ_CP119746:1923511-1923765 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NWK6

Antitoxin

Source ID Structure
AlphaFold DB L4JJX7

References