Detailed information of TA system TA022419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 904615..905320
• Replicon: chromosome
• Accession: NC_009800
• Organism: Escherichia coli HS

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: ECHS_RS04380
• Protein ID: WP_000539521.1
• Coordinates: 904934..905320 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ECHS_RS04375
• Protein ID: WP_001280945.1
• Coordinates: 904615..904944 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECHS_RS04360 (EcHS_A0890)	899772..900683	+	912	WP_001236046.1	glutathione ABC transporter permease GsiD	-
ECHS_RS04365 (EcHS_A0891)	900861..903209	+	2349	WP_012135849.1	EAL domain-containing protein	-
ECHS_RS04370 (EcHS_A0892)	903217..904545	+	1329	WP_000086877.1	GGDEF domain-containing protein	-
ECHS_RS04375 (EcHS_A0893)	904615..904944	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
ECHS_RS04380 (EcHS_A0894)	904934..905320	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECHS_RS04385 (EcHS_A0895)	905546..906871	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
ECHS_RS04390 (EcHS_A0896)	907084..907467	+	384	WP_000497137.1	biofilm formation regulator BssR	-
ECHS_RS04395 (EcHS_A0897)	907578..908693	+	1116	Protein_834	aldose sugar dehydrogenase YliI	-
ECHS_RS04400 (EcHS_A0899)	908690..909316	-	627	WP_012135850.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T22419 WP_000539521.1 NC_009800:c905320-904934 [Escherichia coli HS]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T22419 NC_009800:c905320-904934 [Escherichia coli HS]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT22419 WP_001280945.1 NC_009800:c904944-904615 [Escherichia coli HS]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT22419 NC_009800:c904944-904615 [Escherichia coli HS]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6