Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3214803..3215587 Replicon chromosome
Accession NZ_CP086624
Organism Escherichia coli strain RIVM_C037791

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag LN354_RS15885 Protein ID WP_000613626.1
Coordinates 3215093..3215587 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag LN354_RS15880 Protein ID WP_001110447.1
Coordinates 3214803..3215096 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN354_RS15870 (3209953) 3209953..3210912 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
LN354_RS15875 (3211485) 3211485..3214670 + 3186 WP_001550951.1 ribonuclease E -
LN354_RS15880 (3214803) 3214803..3215096 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
LN354_RS15885 (3215093) 3215093..3215587 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
LN354_RS15890 (3215682) 3215682..3216635 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
LN354_RS15895 (3216647) 3216647..3218290 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
LN354_RS15900 (3218356) 3218356..3219297 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
LN354_RS15905 (3219297) 3219297..3220394 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T224188 WP_000613626.1 NZ_CP086624:3215093-3215587 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T224188 NZ_CP119746:288572-288847 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT224188 WP_001110447.1 NZ_CP086624:3214803-3215096 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT224188 NZ_CP119746:288844-289203 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References