Detailed information of TA system TA224187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2838157..2838795
• Replicon: chromosome
• Accession: NZ_CP086624
• Organism: Escherichia coli strain RIVM_C037791

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: LN354_RS13930
• Protein ID: WP_000813795.1
• Coordinates: 2838619..2838795 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LN354_RS13925
• Protein ID: WP_076797675.1
• Coordinates: 2838157..2838573 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN354_RS13905 (2833309)	2833309..2834250	-	942	WP_001251315.1	ABC transporter permease	-
LN354_RS13910 (2834251)	2834251..2835264	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
LN354_RS13915 (2835282)	2835282..2836427	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
LN354_RS13920 (2836672)	2836672..2838078	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
LN354_RS13925 (2838157)	2838157..2838573	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LN354_RS13930 (2838619)	2838619..2838795	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LN354_RS13935 (2839017)	2839017..2839247	+	231	WP_023910283.1	YncJ family protein	-
LN354_RS13940 (2839339)	2839339..2841300	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LN354_RS13945 (2841373)	2841373..2841909	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
LN354_RS13950 (2842001)	2842001..2843173	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T224187 WP_000813795.1 NZ_CP086624:c2838795-2838619 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T224187 NZ_CP119745:55928-56215 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGATAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACAAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAAAATGAGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT224187 WP_076797675.1 NZ_CP086624:c2838573-2838157 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT224187 NZ_CP119745:55680-55931 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCTTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1