Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1082649..1083303 Replicon chromosome
Accession NZ_CP086624
Organism Escherichia coli strain RIVM_C037791

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LN354_RS05295 Protein ID WP_000244781.1
Coordinates 1082896..1083303 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0V9GG32
Locus tag LN354_RS05290 Protein ID WP_001564007.1
Coordinates 1082649..1082915 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN354_RS05270 (1078737) 1078737..1080170 - 1434 WP_001564009.1 6-phospho-beta-glucosidase BglA -
LN354_RS05275 (1080215) 1080215..1080526 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
LN354_RS05280 (1080690) 1080690..1081349 + 660 WP_000250274.1 hemolysin III family protein -
LN354_RS05285 (1081426) 1081426..1082406 - 981 WP_001564008.1 tRNA-modifying protein YgfZ -
LN354_RS05290 (1082649) 1082649..1082915 + 267 WP_001564007.1 FAD assembly factor SdhE Antitoxin
LN354_RS05295 (1082896) 1082896..1083303 + 408 WP_000244781.1 protein YgfX Toxin
LN354_RS05300 (1083343) 1083343..1083864 - 522 WP_001055874.1 flavodoxin FldB -
LN354_RS05305 (1083976) 1083976..1084872 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LN354_RS05310 (1084897) 1084897..1085607 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LN354_RS05315 (1085613) 1085613..1087346 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T224175 WP_000244781.1 NZ_CP086624:1082896-1083303 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T224175 NZ_CP119740:c3279102-3278725 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGAAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGATGCAGTGAATTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10565.15 Da        Isoelectric Point: 5.1599

>AT224175 WP_001564007.1 NZ_CP086624:1082649-1082915 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWMMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT224175 NZ_CP119740:c3279523-3279149 [Escherichia coli]
GTGTCAGACACACTCCCCAGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGACATCAGCATACCGCCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCACCCGCAACCACTTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9GG32

References