Detailed information of TA system TA224173

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 882193..883028
• Replicon: chromosome
• Accession: NZ_CP086624
• Organism: Escherichia coli strain RIVM_C037791

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0A1A9A5
• Locus tag: LN354_RS04240
• Protein ID: WP_001564063.1
• Coordinates: 882193..882570 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0K5N986
• Locus tag: LN354_RS04245
• Protein ID: WP_021553056.1
• Coordinates: 882660..883028 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN354_RS04210 (877321)	877321..878469	-	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-
LN354_RS04215 (878541)	878541..879524	-	984	WP_001296394.1	KpsF/GutQ family sugar-phosphate isomerase	-
LN354_RS04220 (880334)	880334..880504	-	171	Protein_828	IS110 family transposase	-
LN354_RS04225 (880847)	880847..881689	-	843	Protein_829	DUF4942 domain-containing protein	-
LN354_RS04230 (881774)	881774..881968	-	195	WP_024181941.1	DUF957 domain-containing protein	-
LN354_RS04235 (882047)	882047..882196	-	150	Protein_831	DUF5983 family protein	-
LN354_RS04240 (882193)	882193..882570	-	378	WP_001564063.1	TA system toxin CbtA family protein	Toxin
LN354_RS04245 (882660)	882660..883028	-	369	WP_021553056.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LN354_RS04250 (883191)	883191..883412	-	222	WP_000692345.1	DUF987 domain-containing protein	-
LN354_RS04255 (883477)	883477..883953	-	477	WP_021553055.1	RadC family protein	-
LN354_RS04260 (883969)	883969..884448	-	480	WP_001564060.1	antirestriction protein	-
LN354_RS04265 (884714)	884714..885532	-	819	WP_001564059.1	DUF932 domain-containing protein	-
LN354_RS04270 (885622)	885622..885855	-	234	WP_001278293.1	DUF905 family protein	-
LN354_RS04275 (885861)	885861..886538	-	678	WP_001564058.1	hypothetical protein	-
LN354_RS04280 (886689)	886689..887369	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX	765165..960208	195043
	-	flank	IS/Tn	-	-	880334..880489	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14094.09 Da
• Isoelectric Point: 7.8045

>T224173 WP_001564063.1 NZ_CP086624:c882570-882193 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T224173 NZ_CP119740:2847027-2847134 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13615.39 Da
• Isoelectric Point: 7.0264

>AT224173 WP_021553056.1 NZ_CP086624:c883028-882660 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT224173 NZ_CP119740:c2846980-2846914 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A0A1A9A5

Antitoxin

• AlphaFold DB: A0A0K5N986