Detailed information of TA system TA022417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 511857..512694
• Replicon: chromosome
• Accession: NC_009800
• Organism: Escherichia coli HS

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: ECHS_RS02480
• Protein ID: WP_000227784.1
• Coordinates: 511857..512399 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: ECHS_RS02485
• Protein ID: WP_001297137.1
• Coordinates: 512383..512694 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECHS_RS02460 (EcHS_A0497)	507396..508307	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
ECHS_RS02465 (EcHS_A0498)	508475..508966	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
ECHS_RS02470 (EcHS_A0499)	509094..510458	-	1365	WP_001000978.1	MFS transporter	-
ECHS_RS02475 (EcHS_A0500)	510866..511801	+	936	WP_024186686.1	sel1 repeat family protein	-
ECHS_RS02480 (EcHS_A0501)	511857..512399	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
ECHS_RS02485 (EcHS_A0502)	512383..512694	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
ECHS_RS02490 (EcHS_A0503)	512879..513769	-	891	WP_000971336.1	heme o synthase	-
ECHS_RS02495 (EcHS_A0504)	513781..514110	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
ECHS_RS02500 (EcHS_A0505)	514110..514724	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
ECHS_RS02505 (EcHS_A0506)	514714..516705	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
ECHS_RS02510 (EcHS_A0507)	516727..517674	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T22417 WP_000227784.1 NC_009800:c512399-511857 [Escherichia coli HS]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T22417 NC_009800:c512399-511857 [Escherichia coli HS]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT22417 WP_001297137.1 NC_009800:c512694-512383 [Escherichia coli HS]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT22417 NC_009800:c512694-512383 [Escherichia coli HS]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3