Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4749228..4749725 Replicon chromosome
Accession NZ_CP086623
Organism Escherichia coli strain RIVM_C037794

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LN358_RS23210 Protein ID WP_001173343.1
Coordinates 4749228..4749401 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LN358_RS23215 Protein ID WP_000398619.1
Coordinates 4749429..4749725 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN358_RS23200 (4745898) 4745898..4747355 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
LN358_RS23205 (4747592) 4747592..4749109 + 1518 WP_001550472.1 lysine--tRNA ligase -
LN358_RS23210 (4749228) 4749228..4749401 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LN358_RS23215 (4749429) 4749429..4749725 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LN358_RS23220 (4749954) 4749954..4750226 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LN358_RS23225 (4750238) 4750238..4750468 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
LN358_RS23230 (4750648) 4750648..4752279 + 1632 WP_001216477.1 sensor histidine kinase -
LN358_RS23235 (4752276) 4752276..4752995 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T224168 WP_001173343.1 NZ_CP086623:c4749401-4749228 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T224168 NZ_CP119740:1965020-1965274 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT224168 WP_000398619.1 NZ_CP086623:c4749725-4749429 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT224168 NZ_CP119740:1964772-1965023 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References