Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4652091..4652686 Replicon chromosome
Accession NZ_CP086623
Organism Escherichia coli strain RIVM_C037794

Toxin (Protein)


Gene name chpB Uniprot ID A0A0V9NWK6
Locus tag LN358_RS22705 Protein ID WP_019842229.1
Coordinates 4652091..4652441 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID L4JJX7
Locus tag LN358_RS22710 Protein ID WP_001223213.1
Coordinates 4652435..4652686 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN358_RS22685 (4647421) 4647421..4648443 - 1023 WP_001550507.1 ABC transporter permease -
LN358_RS22690 (4648457) 4648457..4649959 - 1503 WP_001550506.1 sugar ABC transporter ATP-binding protein -
LN358_RS22695 (4650091) 4650091..4651047 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
LN358_RS22700 (4651357) 4651357..4651887 + 531 WP_000055075.1 inorganic diphosphatase -
LN358_RS22705 (4652091) 4652091..4652441 - 351 WP_019842229.1 endoribonuclease toxin ChpB Toxin
LN358_RS22710 (4652435) 4652435..4652686 - 252 WP_001223213.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
LN358_RS22715 (4652898) 4652898..4653239 - 342 WP_001550504.1 gamma-glutamylcyclotransferase -
LN358_RS22720 (4653242) 4653242..4657021 - 3780 WP_001550503.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-115)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12483.44 Da        Isoelectric Point: 5.6097

>T224167 WP_019842229.1 NZ_CP086623:c4652441-4652091 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVWMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Download         Length: 351 bp

>T224167 NZ_CP119740:1964772-1965023 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9331.64 Da        Isoelectric Point: 4.6509

>AT224167 WP_001223213.1 NZ_CP086623:c4652686-4652435 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT224167 NZ_CP119740:1965020-1965274 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NWK6

Antitoxin

Source ID Structure
AlphaFold DB L4JJX7

References