Detailed information of TA system TA224164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4144756..4146445
• Replicon: chromosome
• Accession: NZ_CP086623
• Organism: Escherichia coli strain RIVM_C037794

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: LN358_RS20255
• Protein ID: WP_000365809.1
• Coordinates: 4144756..4145676 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LN358_RS20260
• Protein ID: WP_001550661.1
• Coordinates: 4145663..4146445 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN358_RS20240 (4140846)	4140846..4141973	-	1128	WP_001550663.1	MFS transporter	-
LN358_RS20245 (4142115)	4142115..4143173	-	1059	WP_122987197.1	alpha/beta hydrolase	-
LN358_RS20250 (4143438)	4143438..4144322	+	885	WP_001119037.1	LysR family transcriptional regulator	-
LN358_RS20255 (4144756)	4144756..4145676	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LN358_RS20260 (4145663)	4145663..4146445	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
LN358_RS20265 (4146761)	4146761..4149970	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
LN358_RS20270 (4150317)	4150317..4150787	+	471	WP_000201185.1	hypothetical protein	-
LN358_RS20275 (4150784)	4150784..4150886	+	103	Protein_3978	helix-turn-helix domain-containing protein	-
LN358_RS20280 (4150978)	4150978..4151136	+	159	Protein_3979	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T224164 WP_000365809.1 NZ_CP086623:c4145676-4144756 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T224164 NZ_CP119740:1091963-1092298 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT224164 WP_001550661.1 NZ_CP086623:c4146445-4145663 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT224164 NZ_CP119740:1091715-1091963 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: V0Z9M0