Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3940087..3940705 Replicon chromosome
Accession NZ_CP086623
Organism Escherichia coli strain RIVM_C037794

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LN358_RS19295 Protein ID WP_001291435.1
Coordinates 3940487..3940705 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LN358_RS19290 Protein ID WP_000344800.1
Coordinates 3940087..3940461 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN358_RS19280 (3935176) 3935176..3936369 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LN358_RS19285 (3936392) 3936392..3939541 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LN358_RS19290 (3940087) 3940087..3940461 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LN358_RS19295 (3940487) 3940487..3940705 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LN358_RS19300 (3940877) 3940877..3941428 + 552 WP_000102564.1 maltose O-acetyltransferase -
LN358_RS19305 (3941544) 3941544..3942014 + 471 WP_000136192.1 YlaC family protein -
LN358_RS19310 (3942178) 3942178..3943728 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LN358_RS19315 (3943770) 3943770..3944123 - 354 WP_085334692.1 DUF1428 family protein -
LN358_RS19325 (3944502) 3944502..3944813 + 312 WP_000409911.1 MGMT family protein -
LN358_RS19330 (3944844) 3944844..3945416 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224163 WP_001291435.1 NZ_CP086623:3940487-3940705 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T224163 NZ_CP119740:947452-947859 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT224163 WP_000344800.1 NZ_CP086623:3940087-3940461 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT224163 NZ_CP119740:947205-947471 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References