Detailed information of TA system TA224162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3487039..3487744
• Replicon: chromosome
• Accession: NZ_CP086623
• Organism: Escherichia coli strain RIVM_C037794

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LN358_RS17180
• Protein ID: WP_000539521.1
• Coordinates: 3487039..3487425 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LN358_RS17185
• Protein ID: WP_001280945.1
• Coordinates: 3487415..3487744 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN358_RS17160 (3483043)	3483043..3483669	+	627	WP_001295292.1	glutathione S-transferase GstB	-
LN358_RS17165 (3483666)	3483666..3484781	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
LN358_RS17170 (3484892)	3484892..3485275	-	384	WP_000497137.1	biofilm formation regulator BssR	-
LN358_RS17175 (3485488)	3485488..3486813	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LN358_RS17180 (3487039)	3487039..3487425	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LN358_RS17185 (3487415)	3487415..3487744	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LN358_RS17190 (3487814)	3487814..3489142	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
LN358_RS17195 (3489150)	3489150..3491498	-	2349	WP_001550886.1	EAL domain-containing protein	-
LN358_RS17200 (3491676)	3491676..3492587	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T224162 WP_000539521.1 NZ_CP086623:3487039-3487425 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T224162 NZ_CP119740:763610-763906 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT224162 WP_001280945.1 NZ_CP086623:3487415-3487744 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT224162 NZ_CP119740:763908-764303 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6