Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1084203..1084857 Replicon chromosome
Accession NZ_CP086623
Organism Escherichia coli strain RIVM_C037794

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LN358_RS05295 Protein ID WP_000244781.1
Coordinates 1084450..1084857 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0V9GG32
Locus tag LN358_RS05290 Protein ID WP_001564007.1
Coordinates 1084203..1084469 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN358_RS05270 (1080291) 1080291..1081724 - 1434 WP_001564009.1 6-phospho-beta-glucosidase BglA -
LN358_RS05275 (1081769) 1081769..1082080 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
LN358_RS05280 (1082244) 1082244..1082903 + 660 WP_000250274.1 hemolysin III family protein -
LN358_RS05285 (1082980) 1082980..1083960 - 981 WP_001564008.1 tRNA-modifying protein YgfZ -
LN358_RS05290 (1084203) 1084203..1084469 + 267 WP_001564007.1 FAD assembly factor SdhE Antitoxin
LN358_RS05295 (1084450) 1084450..1084857 + 408 WP_000244781.1 protein YgfX Toxin
LN358_RS05300 (1084897) 1084897..1085418 - 522 WP_001055874.1 flavodoxin FldB -
LN358_RS05305 (1085530) 1085530..1086426 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LN358_RS05310 (1086451) 1086451..1087161 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LN358_RS05315 (1087167) 1087167..1088900 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T224148 WP_000244781.1 NZ_CP086623:1084450-1084857 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T224148 NZ_CP119734:4634661-4635038 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10565.15 Da        Isoelectric Point: 5.1599

>AT224148 WP_001564007.1 NZ_CP086623:1084203-1084469 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWMMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT224148 NZ_CP119734:4634237-4634614 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9GG32

References