Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 958439..959234 | Replicon | chromosome |
| Accession | NZ_CP086623 | ||
| Organism | Escherichia coli strain RIVM_C037794 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A4Y9BN12 |
| Locus tag | LN358_RS04650 | Protein ID | WP_023909482.1 |
| Coordinates | 958439..958813 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A4Y9BHM8 |
| Locus tag | LN358_RS04655 | Protein ID | WP_023909483.1 |
| Coordinates | 958860..959234 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LN358_RS04620 (954404) | 954404..955669 | - | 1266 | WP_001218869.1 | integrase arm-type DNA-binding domain-containing protein | - |
| LN358_RS04625 (956133) | 956133..956459 | - | 327 | WP_001522265.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LN358_RS04630 (956456) | 956456..956719 | - | 264 | WP_001143297.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| LN358_RS04635 (956791) | 956791..957657 | - | 867 | WP_023909479.1 | DUF4942 domain-containing protein | - |
| LN358_RS04640 (957742) | 957742..957939 | - | 198 | WP_001522268.1 | DUF957 domain-containing protein | - |
| LN358_RS04645 (957951) | 957951..958442 | - | 492 | WP_023909481.1 | DUF5983 family protein | - |
| LN358_RS04650 (958439) | 958439..958813 | - | 375 | WP_023909482.1 | TA system toxin CbtA family protein | Toxin |
| LN358_RS04655 (958860) | 958860..959234 | - | 375 | WP_023909483.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| LN358_RS04660 (959314) | 959314..959535 | - | 222 | WP_023909484.1 | DUF987 domain-containing protein | - |
| LN358_RS04665 (959587) | 959587..960342 | - | 756 | WP_001522280.1 | IS21-like element ISEc10 family helper ATPase IstB | - |
| LN358_RS04670 (960339) | 960339..961838 | - | 1500 | WP_059332257.1 | IS21-like element ISEc10 family transposase | - |
| LN358_RS04675 (962019) | 962019..962495 | - | 477 | WP_001186724.1 | RadC family protein | - |
| LN358_RS04680 (962510) | 962510..962983 | - | 474 | WP_001522284.1 | antirestriction protein | - |
| LN358_RS04685 (963078) | 963078..963254 | - | 177 | WP_001522285.1 | hypothetical protein | - |
| LN358_RS04690 (963254) | 963254..964072 | - | 819 | WP_001522287.1 | DUF932 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX | 765942..961762 | 195820 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14009.98 Da Isoelectric Point: 8.2905
>T224147 WP_023909482.1 NZ_CP086623:c958813-958439 [Escherichia coli]
MKTLSDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRTVNNITQGKHPEAK
MKTLSDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRTVNNITQGKHPEAK
Download Length: 375 bp
>T224147 NZ_CP119734:4615941-4616318 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13598.31 Da Isoelectric Point: 5.5051
>AT224147 WP_023909483.1 NZ_CP086623:c959234-958860 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT224147 NZ_CP119734:4615520-4615894 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y9BN12 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y9BHM8 |