Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 883747..884582 Replicon chromosome
Accession NZ_CP086623
Organism Escherichia coli strain RIVM_C037794

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0A1A9A5
Locus tag LN358_RS04240 Protein ID WP_001564063.1
Coordinates 883747..884124 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0K5N986
Locus tag LN358_RS04245 Protein ID WP_021553056.1
Coordinates 884214..884582 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN358_RS04210 (878875) 878875..880023 - 1149 WP_000905920.1 capsule polysaccharide export inner-membrane protein KpsE -
LN358_RS04215 (880095) 880095..881078 - 984 WP_001296394.1 KpsF/GutQ family sugar-phosphate isomerase -
LN358_RS04220 (881888) 881888..882058 - 171 Protein_828 IS110 family transposase -
LN358_RS04225 (882401) 882401..883243 - 843 Protein_829 DUF4942 domain-containing protein -
LN358_RS04230 (883328) 883328..883522 - 195 WP_024181941.1 DUF957 domain-containing protein -
LN358_RS04235 (883601) 883601..883750 - 150 Protein_831 DUF5983 family protein -
LN358_RS04240 (883747) 883747..884124 - 378 WP_001564063.1 TA system toxin CbtA family protein Toxin
LN358_RS04245 (884214) 884214..884582 - 369 WP_021553056.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LN358_RS04250 (884745) 884745..884966 - 222 WP_000692345.1 DUF987 domain-containing protein -
LN358_RS04255 (885031) 885031..885507 - 477 WP_021553055.1 RadC family protein -
LN358_RS04260 (885523) 885523..886002 - 480 WP_001564060.1 antirestriction protein -
LN358_RS04265 (886268) 886268..887086 - 819 WP_001564059.1 DUF932 domain-containing protein -
LN358_RS04270 (887176) 887176..887409 - 234 WP_001278293.1 DUF905 family protein -
LN358_RS04275 (887415) 887415..888092 - 678 WP_229319006.1 hypothetical protein -
LN358_RS04280 (888243) 888243..888923 - 681 WP_001278649.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX 765942..961762 195820
- flank IS/Tn - - 881888..882043 155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14094.09 Da        Isoelectric Point: 7.8045

>T224146 WP_001564063.1 NZ_CP086623:c884124-883747 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T224146 NZ_CP119734:3780693-3780911 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13615.39 Da        Isoelectric Point: 7.0264

>AT224146 WP_021553056.1 NZ_CP086623:c884582-884214 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT224146 NZ_CP119734:3780293-3780667 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A9A5

Antitoxin

Source ID Structure
AlphaFold DB A0A0K5N986

References