Detailed information of TA system TA224144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 207428..208040
• Replicon: chromosome
• Accession: NZ_CP086623
• Organism: Escherichia coli strain RIVM_C037794

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: LN358_RS00945
• Protein ID: WP_000833473.1
• Coordinates: 207428..207613 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: LN358_RS00950
• Protein ID: WP_000499744.1
• Coordinates: 207630..208040 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN358_RS00935 (204269)	204269..205420	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
LN358_RS00940 (205621)	205621..207159	+	1539	WP_001551836.1	aldehyde dehydrogenase AldB	-
LN358_RS00945 (207428)	207428..207613	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
LN358_RS00950 (207630)	207630..208040	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LN358_RS00955 (208112)	208112..210076	-	1965	WP_001551835.1	glycoside hydrolase family 127 protein	-
LN358_RS00960 (210087)	210087..211487	-	1401	WP_000204811.1	MFS transporter	-
LN358_RS00965 (211713)	211713..212528	+	816	WP_000891823.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T224144 WP_000833473.1 NZ_CP086623:207428-207613 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T224144 NZ_CP119734:c3129123-3128716 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT224144 WP_000499744.1 NZ_CP086623:207630-208040 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT224144 NZ_CP119734:c3129370-3129104 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9