Detailed information of TA system TA224143

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 72584..73416
• Replicon: chromosome
• Accession: NZ_CP086623
• Organism: Escherichia coli strain RIVM_C037794

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E3PN67
• Locus tag: LN358_RS00310
• Protein ID: WP_000854775.1
• Coordinates: 72584..72958 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A426P7N5
• Locus tag: LN358_RS00315
• Protein ID: WP_001285645.1
• Coordinates: 73048..73416 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN358_RS00280 (67948)	67948..68871	-	924	WP_000535949.1	carboxylate/amino acid/amine transporter	-
LN358_RS00285 (68982)	68982..70166	-	1185	WP_001172876.1	sugar efflux transporter	-
LN358_RS00290 (70571)	70571..70776	-	206	Protein_57	RhuM family protein	-
LN358_RS00295 (70960)	70960..71802	-	843	WP_001274548.1	DUF4942 domain-containing protein	-
LN358_RS00300 (71887)	71887..72084	-	198	WP_000772035.1	DUF957 domain-containing protein	-
LN358_RS00305 (72096)	72096..72587	-	492	WP_000976825.1	DUF5983 family protein	-
LN358_RS00310 (72584)	72584..72958	-	375	WP_000854775.1	TA system toxin CbtA family protein	Toxin
LN358_RS00315 (73048)	73048..73416	-	369	WP_001285645.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LN358_RS00320 (73579)	73579..73800	-	222	WP_000692303.1	DUF987 domain-containing protein	-
LN358_RS00325 (73869)	73869..74345	-	477	WP_001318130.1	RadC family protein	-
LN358_RS00330 (74361)	74361..74846	-	486	WP_000213721.1	antirestriction protein	-
LN358_RS00335 (74938)	74938..75756	-	819	WP_001175173.1	DUF932 domain-containing protein	-
LN358_RS00340 (75846)	75846..76079	-	234	WP_001278284.1	DUF905 family protein	-
LN358_RS00345 (76085)	76085..76762	-	678	WP_001097567.1	hypothetical protein	-
LN358_RS00350 (76913)	76913..77593	-	681	WP_001282911.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaOXA-244	-	61259..123838	62579

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13965.02 Da
• Isoelectric Point: 8.2830

>T224143 WP_000854775.1 NZ_CP086623:c72958-72584 [Escherichia coli]
MKTLPDTLVREASRCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T224143 NZ_CP119734:c2984605-2984270 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13678.37 Da
• Isoelectric Point: 5.4492

>AT224143 WP_001285645.1 NZ_CP086623:c73416-73048 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTNG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT224143 NZ_CP119734:c2984853-2984605 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: E3PN67

Antitoxin

• AlphaFold DB: A0A426P7N5