Detailed information of TA system TA022414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2890364..2890980
• Replicon: chromosome
• Accession: NC_009792
• Organism: Citrobacter koseri ATCC BAA-895

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A8AL49
• Locus tag: CKO_RS13330
• Protein ID: WP_012133919.1
• Coordinates: 2890364..2890738 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CKO_RS13335
• Protein ID: WP_160880417.1
• Coordinates: 2890738..2890980 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKO_RS13315	2887867..2888769	+	903	WP_012133916.1	formate dehydrogenase O subunit beta	-
CKO_RS13320	2888766..2889401	+	636	WP_012133917.1	formate dehydrogenase cytochrome b556 subunit	-
CKO_RS13325	2889398..2890327	+	930	WP_012133918.1	formate dehydrogenase accessory protein FdhE	-
CKO_RS13330	2890364..2890738	-	375	WP_012133919.1	type II toxin-antitoxin system VapC family toxin	Toxin
CKO_RS13335	2890738..2890980	-	243	WP_160880417.1	ribbon-helix-helix domain-containing protein	Antitoxin
CKO_RS13340	2891216..2892157	-	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
CKO_RS13345	2892202..2892639	-	438	WP_012133923.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CKO_RS13350	2892636..2893508	-	873	WP_012133924.1	virulence factor BrkB family protein	-
CKO_RS13355	2893502..2894101	-	600	WP_024130671.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13741.00 Da
• Isoelectric Point: 9.8061

>T22414 WP_012133919.1 NC_009792:c2890738-2890364 [Citrobacter koseri ATCC BAA-895]
MIKGPVLFDTNILIDLFSGRGEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T22414 NC_009792:c2890738-2890364 [Citrobacter koseri ATCC BAA-895]
ATGATTAAAGGTCCGGTGTTGTTTGACACCAATATTCTCATTGATTTATTTAGCGGGCGTGGTGAAGCGAAGAAGGCTCT
CGAAGCTTACTCACCCCAGAATGCCATTAGTCTCATTACCTGGATGGAAGTGATGGTAGGGGCAAAAAAGTACCATCAGG
AATCCCGCACACGTATAGCATTGAGCGCTTTTAACGTTATCGGCGTTTCACAGGATATTGCTGAAAGAAGTGTCAGTCTC
AGGCAGGAATACGGGATGAAGTTGCCGGATGCGGTCATTCTGGCTACAGCGCACATTCATCGTTTTGCGCTCGTGACGCG
CAATACAAAGGACTTTGCGGGGATTCCGGGAGTCATTACGCCTTACCATTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9396.62 Da
• Isoelectric Point: 4.1961

>AT22414 WP_160880417.1 NC_009792:c2890980-2890738 [Citrobacter koseri ATCC BAA-895]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW

Nucleotide

• Length: 243 bp

>AT22414 NC_009792:c2890980-2890738 [Citrobacter koseri ATCC BAA-895]
ATGATGGCTGGGATGGAAATGGGCAGAATATTACTTGATTTATCAGATGAAGTTATCAAGCGGCTTGATGACCTCAAACA
ACAGCGTAATCTTCCGCGAGCAGAGCTATTACGCGAAGCAGTTGAACAATACCTGGAAAGGCAGGGACAAGCCGAAACCA
CTATTTCCGATGCGCTGGGGCTCTGGCAAGGTTGCGAAGAAGATGGCGTTGAATACGAAAGGAAACTTCGCGAGGAGTGG
TGA

Structures

Toxin

• AlphaFold DB: A8AL49