Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4512275..4512851 Replicon chromosome
Accession NZ_CP086622
Organism Escherichia coli strain RIVM_C037884

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LN352_RS22005 Protein ID WP_001297643.1
Coordinates 4512564..4512851 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag LN352_RS22000 Protein ID WP_000063160.1
Coordinates 4512275..4512577 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN352_RS21985 (4508871) 4508871..4511021 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
LN352_RS21990 (4511071) 4511071..4511274 + 204 WP_000467859.1 YbdD/YjiX family protein -
LN352_RS21995 (4511285) 4511285..4512241 + 957 WP_001297640.1 GTPase -
LN352_RS22000 (4512275) 4512275..4512577 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
LN352_RS22005 (4512564) 4512564..4512851 - 288 WP_001297643.1 BrnT family toxin Toxin
LN352_RS25610 (4513050) 4513050..4513746 - 697 Protein_4314 IS1 family transposase -
LN352_RS22020 (4514061) 4514061..4515293 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
LN352_RS22025 (4515334) 4515334..4516614 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4513050..4513259 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T224139 WP_001297643.1 NZ_CP086622:c4512851-4512564 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T224139 NZ_CP119734:c2111551-2111297 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT224139 WP_000063160.1 NZ_CP086622:c4512577-4512275 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT224139 NZ_CP119734:c2111799-2111548 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References