Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3954411..3955029 Replicon chromosome
Accession NZ_CP086622
Organism Escherichia coli strain RIVM_C037884

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LN352_RS19380 Protein ID WP_001291435.1
Coordinates 3954811..3955029 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LN352_RS19375 Protein ID WP_000344800.1
Coordinates 3954411..3954785 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN352_RS19365 (3949500) 3949500..3950693 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LN352_RS19370 (3950716) 3950716..3953865 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LN352_RS19375 (3954411) 3954411..3954785 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LN352_RS19380 (3954811) 3954811..3955029 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LN352_RS19385 (3955201) 3955201..3955752 + 552 WP_000102564.1 maltose O-acetyltransferase -
LN352_RS19390 (3955868) 3955868..3956338 + 471 WP_000136192.1 YlaC family protein -
LN352_RS19395 (3956502) 3956502..3958052 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LN352_RS19400 (3958094) 3958094..3958447 - 354 WP_085334692.1 DUF1428 family protein -
LN352_RS19410 (3958826) 3958826..3959137 + 312 WP_000409911.1 MGMT family protein -
LN352_RS19415 (3959168) 3959168..3959740 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224136 WP_001291435.1 NZ_CP086622:3954811-3955029 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T224136 NZ_CP119734:c1612108-1611821 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT224136 WP_000344800.1 NZ_CP086622:3954411-3954785 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT224136 NZ_CP119734:c1612347-1612108 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References