Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3245971..3246755 Replicon chromosome
Accession NZ_CP086622
Organism Escherichia coli strain RIVM_C037884

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag LN352_RS16055 Protein ID WP_000613626.1
Coordinates 3246261..3246755 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag LN352_RS16050 Protein ID WP_001110447.1
Coordinates 3245971..3246264 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN352_RS16040 (3241121) 3241121..3242080 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
LN352_RS16045 (3242653) 3242653..3245838 + 3186 WP_001550951.1 ribonuclease E -
LN352_RS16050 (3245971) 3245971..3246264 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
LN352_RS16055 (3246261) 3246261..3246755 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
LN352_RS16060 (3246850) 3246850..3247803 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
LN352_RS16065 (3247815) 3247815..3249458 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
LN352_RS16070 (3249524) 3249524..3250465 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
LN352_RS16075 (3250465) 3250465..3251562 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T224134 WP_000613626.1 NZ_CP086622:3246261-3246755 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T224134 NZ_CP119734:c1229544-1229437 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT224134 WP_001110447.1 NZ_CP086622:3245971-3246264 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT224134 NZ_CP119734:1229591-1229657 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References