Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1082585..1083239 Replicon chromosome
Accession NZ_CP086622
Organism Escherichia coli strain RIVM_C037884

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LN352_RS05280 Protein ID WP_000244781.1
Coordinates 1082832..1083239 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0V9GG32
Locus tag LN352_RS05275 Protein ID WP_001564007.1
Coordinates 1082585..1082851 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN352_RS05255 (1078673) 1078673..1080106 - 1434 WP_001564009.1 6-phospho-beta-glucosidase BglA -
LN352_RS05260 (1080151) 1080151..1080462 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
LN352_RS05265 (1080626) 1080626..1081285 + 660 WP_000250274.1 hemolysin III family protein -
LN352_RS05270 (1081362) 1081362..1082342 - 981 WP_001564008.1 tRNA-modifying protein YgfZ -
LN352_RS05275 (1082585) 1082585..1082851 + 267 WP_001564007.1 FAD assembly factor SdhE Antitoxin
LN352_RS05280 (1082832) 1082832..1083239 + 408 WP_000244781.1 protein YgfX Toxin
LN352_RS05285 (1083279) 1083279..1083800 - 522 WP_001055874.1 flavodoxin FldB -
LN352_RS05290 (1083912) 1083912..1084808 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LN352_RS05295 (1084833) 1084833..1085543 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LN352_RS05300 (1085549) 1085549..1087282 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T224121 WP_000244781.1 NZ_CP086622:1082832-1083239 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T224121 NZ_CP119730:c4790935-4790624 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACCGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10565.15 Da        Isoelectric Point: 5.1599

>AT224121 WP_001564007.1 NZ_CP086622:1082585-1082851 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWMMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT224121 NZ_CP119730:c4790623-4790333 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9GG32

References