Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 956821..957616 | Replicon | chromosome |
| Accession | NZ_CP086622 | ||
| Organism | Escherichia coli strain RIVM_C037884 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A4Y9BN12 |
| Locus tag | LN352_RS04635 | Protein ID | WP_023909482.1 |
| Coordinates | 956821..957195 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A4Y9BHM8 |
| Locus tag | LN352_RS04640 | Protein ID | WP_023909483.1 |
| Coordinates | 957242..957616 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LN352_RS04605 (952786) | 952786..954051 | - | 1266 | WP_001218869.1 | integrase arm-type DNA-binding domain-containing protein | - |
| LN352_RS04610 (954515) | 954515..954841 | - | 327 | WP_001522265.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LN352_RS04615 (954838) | 954838..955101 | - | 264 | WP_001143297.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| LN352_RS04620 (955173) | 955173..956039 | - | 867 | WP_023909479.1 | DUF4942 domain-containing protein | - |
| LN352_RS04625 (956124) | 956124..956321 | - | 198 | WP_001522268.1 | DUF957 domain-containing protein | - |
| LN352_RS04630 (956333) | 956333..956824 | - | 492 | WP_023909481.1 | DUF5983 family protein | - |
| LN352_RS04635 (956821) | 956821..957195 | - | 375 | WP_023909482.1 | TA system toxin CbtA family protein | Toxin |
| LN352_RS04640 (957242) | 957242..957616 | - | 375 | WP_023909483.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| LN352_RS04645 (957696) | 957696..957917 | - | 222 | WP_023909484.1 | DUF987 domain-containing protein | - |
| LN352_RS04650 (957969) | 957969..958724 | - | 756 | WP_001522280.1 | IS21-like element ISEc10 family helper ATPase IstB | - |
| LN352_RS04655 (958721) | 958721..960220 | - | 1500 | WP_059332257.1 | IS21-like element ISEc10 family transposase | - |
| LN352_RS04660 (960401) | 960401..960877 | - | 477 | WP_001186724.1 | RadC family protein | - |
| LN352_RS04665 (960892) | 960892..961365 | - | 474 | WP_001522284.1 | antirestriction protein | - |
| LN352_RS04670 (961460) | 961460..961636 | - | 177 | WP_001522285.1 | hypothetical protein | - |
| LN352_RS04675 (961636) | 961636..962454 | - | 819 | WP_001522287.1 | DUF932 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX | 765101..960144 | 195043 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14009.98 Da Isoelectric Point: 8.2905
>T224120 WP_023909482.1 NZ_CP086622:c957195-956821 [Escherichia coli]
MKTLSDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRTVNNITQGKHPEAK
MKTLSDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRTVNNITQGKHPEAK
Download Length: 375 bp
>T224120 NZ_CP119730:c4509773-4509600 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG
Antitoxin
Download Length: 125 a.a. Molecular weight: 13598.31 Da Isoelectric Point: 5.5051
>AT224120 WP_023909483.1 NZ_CP086622:c957616-957242 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT224120 NZ_CP119730:c4510097-4509801 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y9BN12 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y9BHM8 |