Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 72094..72619 Replicon plasmid pRIVM_C039205_2
Accession NZ_CP086621
Organism Escherichia coli strain RIVM_C039205

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag LN351_RS25840 Protein ID WP_001159871.1
Coordinates 72314..72619 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A4D9F4P3
Locus tag LN351_RS25835 Protein ID WP_032256270.1
Coordinates 72094..72312 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN351_RS25830 (69131) 69131..69772 - 642 WP_000990667.1 hypothetical protein -
LN351_RS25835 (72094) 72094..72312 + 219 WP_032256270.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LN351_RS25840 (72314) 72314..72619 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
LN351_RS25845 (72620) 72620..73429 + 810 WP_000016979.1 site-specific integrase -
LN351_RS25850 (73567) 73567..73842 - 276 WP_000239529.1 hypothetical protein -
LN351_RS25855 (73836) 73836..74480 - 645 WP_000633911.1 AAA family ATPase -
LN351_RS25860 (74709) 74709..75680 + 972 WP_001103694.1 plasmid segregation protein ParM -
LN351_RS25865 (75685) 75685..76077 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
LN351_RS25870 (76082) 76082..76333 - 252 Protein_96 DUF4113 domain-containing protein -
LN351_RS25875 (76395) 76395..77192 - 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 - 1..135854 135854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T224112 WP_001159871.1 NZ_CP086621:72314-72619 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T224112 NZ_CP119730:c2613941-2613765 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8388.34 Da        Isoelectric Point: 4.7405

>AT224112 WP_032256270.1 NZ_CP086621:72094-72312 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMSEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT224112 NZ_CP119730:c2613719-2613303 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A4D9F4P3

References