Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4732268..4732765 Replicon chromosome
Accession NZ_CP086620
Organism Escherichia coli strain RIVM_C039205

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LN351_RS23110 Protein ID WP_001173343.1
Coordinates 4732268..4732441 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LN351_RS23115 Protein ID WP_000398619.1
Coordinates 4732469..4732765 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN351_RS23100 (4728938) 4728938..4730395 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
LN351_RS23105 (4730632) 4730632..4732149 + 1518 WP_001550472.1 lysine--tRNA ligase -
LN351_RS23110 (4732268) 4732268..4732441 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LN351_RS23115 (4732469) 4732469..4732765 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LN351_RS23120 (4732994) 4732994..4733266 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LN351_RS23125 (4733278) 4733278..4733508 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
LN351_RS23130 (4733688) 4733688..4735319 + 1632 WP_001216477.1 sensor histidine kinase -
LN351_RS23135 (4735316) 4735316..4736035 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T224109 WP_001173343.1 NZ_CP086620:c4732441-4732268 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T224109 NZ_CP119730:1923511-1923765 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT224109 WP_000398619.1 NZ_CP086620:c4732765-4732469 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT224109 NZ_CP119730:1923263-1923514 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References