Detailed information of TA system TA224101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2838197..2838835
• Replicon: chromosome
• Accession: NZ_CP086620
• Organism: Escherichia coli strain RIVM_C039205

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: LN351_RS13920
• Protein ID: WP_000813795.1
• Coordinates: 2838659..2838835 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LN351_RS13915
• Protein ID: WP_076797675.1
• Coordinates: 2838197..2838613 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN351_RS13895 (2833349)	2833349..2834290	-	942	WP_001251315.1	ABC transporter permease	-
LN351_RS13900 (2834291)	2834291..2835304	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
LN351_RS13905 (2835322)	2835322..2836467	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
LN351_RS13910 (2836712)	2836712..2838118	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
LN351_RS13915 (2838197)	2838197..2838613	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LN351_RS13920 (2838659)	2838659..2838835	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LN351_RS13925 (2839057)	2839057..2839287	+	231	WP_023910283.1	YncJ family protein	-
LN351_RS13930 (2839379)	2839379..2841340	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LN351_RS13935 (2841413)	2841413..2841949	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
LN351_RS13940 (2842041)	2842041..2843213	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T224101 WP_000813795.1 NZ_CP086620:c2838835-2838659 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T224101 NZ_CP119729:101678-101983 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT224101 WP_076797675.1 NZ_CP086620:c2838613-2838197 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT224101 NZ_CP119729:101458-101676 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1