Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 67243..67768 Replicon plasmid pRIVM_C039227_1
Accession NZ_CP086619
Organism Escherichia coli strain RIVM_C039227

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag LN355_RS25645 Protein ID WP_001159871.1
Coordinates 67463..67768 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag LN355_RS25640 Protein ID WP_000813634.1
Coordinates 67243..67461 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN355_RS25635 (64280) 64280..64921 - 642 WP_000990667.1 hypothetical protein -
LN355_RS25640 (67243) 67243..67461 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LN355_RS25645 (67463) 67463..67768 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
LN355_RS25650 (67769) 67769..68578 + 810 WP_000016979.1 site-specific integrase -
LN355_RS25655 (68716) 68716..68991 - 276 WP_000239529.1 hypothetical protein -
LN355_RS25660 (68985) 68985..69629 - 645 WP_000633911.1 AAA family ATPase -
LN355_RS25665 (69858) 69858..70829 + 972 WP_001103694.1 plasmid segregation protein ParM -
LN355_RS25670 (70834) 70834..71226 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
LN355_RS25675 (71231) 71231..71482 - 252 Protein_92 DUF4113 domain-containing protein -
LN355_RS25680 (71544) 71544..72341 - 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) - 1..126075 126075


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T224081 WP_001159871.1 NZ_CP086619:67463-67768 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T224081 NZ_CP119726:1407382-1407780 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT224081 WP_000813634.1 NZ_CP086619:67243-67461 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT224081 NZ_CP119726:1407155-1407382 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References