Detailed information of TA system TA224080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 57375..57934
• Replicon: plasmid pRIVM_C039227_1
• Accession: NZ_CP086619
• Organism: Escherichia coli strain RIVM_C039227

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G4VUF8
• Locus tag: LN355_RS25590
• Protein ID: WP_000421273.1
• Coordinates: 57659..57934 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: LN355_RS25585
• Protein ID: WP_001387467.1
• Coordinates: 57375..57659 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN355_RS25560 (52760)	52760..53326	-	567	Protein_69	zinc-binding dehydrogenase	-
LN355_RS25565 (53679)	53679..54569	+	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
LN355_RS25570 (54594)	54594..54974	+	381	WP_000710783.1	Rid family detoxifying hydrolase	-
LN355_RS25575 (55007)	55007..55972	+	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
LN355_RS25580 (56018)	56018..56770	-	753	WP_001403368.1	IclR family transcriptional regulator	-
LN355_RS25585 (57375)	57375..57659	+	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
LN355_RS25590 (57659)	57659..57934	+	276	WP_000421273.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LN355_RS25595 (58040)	58040..58243	+	204	Protein_76	hypothetical protein	-
LN355_RS25600 (58368)	58368..58496	-	129	Protein_77	IS481 family transposase	-
LN355_RS25605 (58529)	58529..59698	-	1170	WP_001403369.1	hypothetical protein	-
LN355_RS25610 (60230)	60230..60498	+	269	Protein_79	type II toxin-antitoxin system ParD family antitoxin	-
LN355_RS25615 (60485)	60485..60775	+	291	Protein_80	type II toxin-antitoxin system RelE/ParE family toxin	-
LN355_RS25620 (60842)	60842..61024	+	183	WP_042344623.1	hypothetical protein	-
LN355_RS25625 (61145)	61145..61885	+	741	WP_001066941.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A)	-	1..126075	126075

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10765.45 Da
• Isoelectric Point: 9.9324

>T224080 WP_000421273.1 NZ_CP086619:57659-57934 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T224080 NZ_CP086619:57659-57934 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTATGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT224080 WP_001387467.1 NZ_CP086619:57375-57659 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT224080 NZ_CP086619:57375-57659 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6H1

Antitoxin

• AlphaFold DB: A0A0E0Y880