Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4711262..4711759 Replicon chromosome
Accession NZ_CP086618
Organism Escherichia coli strain RIVM_C039227

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LN355_RS22935 Protein ID WP_001173343.1
Coordinates 4711262..4711435 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LN355_RS22940 Protein ID WP_000398619.1
Coordinates 4711463..4711759 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN355_RS22925 (4707932) 4707932..4709389 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
LN355_RS22930 (4709626) 4709626..4711143 + 1518 WP_001550472.1 lysine--tRNA ligase -
LN355_RS22935 (4711262) 4711262..4711435 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LN355_RS22940 (4711463) 4711463..4711759 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LN355_RS22945 (4711988) 4711988..4712260 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LN355_RS22950 (4712272) 4712272..4712502 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
LN355_RS22955 (4712682) 4712682..4714313 + 1632 WP_001216477.1 sensor histidine kinase -
LN355_RS22960 (4714310) 4714310..4715029 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T224078 WP_001173343.1 NZ_CP086618:c4711435-4711262 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T224078 NZ_CP119726:c781003-780626 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT224078 WP_000398619.1 NZ_CP086618:c4711759-4711463 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT224078 NZ_CP119726:c781424-781050 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References