Detailed information of TA system TA224074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4106770..4108459
• Replicon: chromosome
• Accession: NZ_CP086618
• Organism: Escherichia coli strain RIVM_C039227

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: LN355_RS19985
• Protein ID: WP_000365809.1
• Coordinates: 4106770..4107690 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LN355_RS19990
• Protein ID: WP_001550661.1
• Coordinates: 4107677..4108459 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN355_RS19970 (4102860)	4102860..4103987	-	1128	WP_001550663.1	MFS transporter	-
LN355_RS19975 (4104129)	4104129..4105187	-	1059	WP_122987197.1	alpha/beta hydrolase	-
LN355_RS19980 (4105452)	4105452..4106336	+	885	WP_001119037.1	LysR family transcriptional regulator	-
LN355_RS19985 (4106770)	4106770..4107690	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LN355_RS19990 (4107677)	4107677..4108459	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
LN355_RS19995 (4108775)	4108775..4111984	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
LN355_RS20000 (4112331)	4112331..4112801	+	471	WP_000201185.1	hypothetical protein	-
LN355_RS20005 (4112798)	4112798..4112900	+	103	Protein_3923	helix-turn-helix domain-containing protein	-
LN355_RS20010 (4112992)	4112992..4113150	+	159	Protein_3924	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T224074 WP_000365809.1 NZ_CP086618:c4107690-4106770 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T224074 NZ_CP119676:1100611-1100892 [Varunaivibrio sulfuroxidans]
ATGATCAAGAGCTTCCACAACAAAGAAACTCAAGCGCTTTTCGAACGAGTGAGGTGCTATCGGAAGTGGCGTTCTTTCGA
GCGTATTGCATTGCGCAAGCTTGTTATGGTGCATGCGGCAAAGGAACTGAAAGACCTGGCGTGTCCCCCGAATAATCGTC
TTGAGGGCCTTAAAGGCGACAGGCAAAGTCAATACTCGATCCGTATCAACCAACAATATCGCGTCTGTTTCGAGTGGAAA
GAAGGGGACGCTTACGACGTGGAGATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT224074 WP_001550661.1 NZ_CP086618:c4108459-4107677 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT224074 NZ_CP119676:1100908-1101189 [Varunaivibrio sulfuroxidans]
ATGATTTTTCCCATTCATCCCGGTGAAATTTTGAAAAATGAATTTATGGAACCTCTCGGCTTGAGTGCGAATAAGCTTGC
CGCCGCCTTAAAGGTGCCGACGAATAGGCTTACCGGGATTGTCGGTGGCGACCGGAGCATTACGGCCGATACCGCTTTGC
GGCTCTCGGACGCCTTCGGCACGACGCCGGAGTTCTGGGTCGGCTTGCAAAATCACTACAATCTGGAAGTGGCCAGGCAG
AAGAAACGGCCTAAGATCGAGAGAATATCGGCGGCGGCATGA

Structures

Toxin

• AlphaFold DB: V0Z9M0