Detailed information of TA system TA224072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3449053..3449758
• Replicon: chromosome
• Accession: NZ_CP086618
• Organism: Escherichia coli strain RIVM_C039227

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LN355_RS16910
• Protein ID: WP_000539521.1
• Coordinates: 3449053..3449439 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LN355_RS16915
• Protein ID: WP_001280945.1
• Coordinates: 3449429..3449758 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN355_RS16890 (3445057)	3445057..3445683	+	627	WP_001295292.1	glutathione S-transferase GstB	-
LN355_RS16895 (3445680)	3445680..3446795	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
LN355_RS16900 (3446906)	3446906..3447289	-	384	WP_000497137.1	biofilm formation regulator BssR	-
LN355_RS16905 (3447502)	3447502..3448827	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LN355_RS16910 (3449053)	3449053..3449439	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LN355_RS16915 (3449429)	3449429..3449758	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LN355_RS16920 (3449828)	3449828..3451156	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
LN355_RS16925 (3451164)	3451164..3453512	-	2349	WP_001550886.1	EAL domain-containing protein	-
LN355_RS16930 (3453690)	3453690..3454601	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T224072 WP_000539521.1 NZ_CP086618:3449053-3449439 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T224072 NZ_CP119675:3447601-3447951 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT224072 WP_001280945.1 NZ_CP086618:3449429-3449758 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT224072 NZ_CP119675:3447314-3447595 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6