Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1082565..1083219 Replicon chromosome
Accession NZ_CP086618
Organism Escherichia coli strain RIVM_C039227

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LN355_RS05285 Protein ID WP_000244781.1
Coordinates 1082812..1083219 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0V9GG32
Locus tag LN355_RS05280 Protein ID WP_001564007.1
Coordinates 1082565..1082831 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN355_RS05260 (1078653) 1078653..1080086 - 1434 WP_001564009.1 6-phospho-beta-glucosidase BglA -
LN355_RS05265 (1080131) 1080131..1080442 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
LN355_RS05270 (1080606) 1080606..1081265 + 660 WP_000250274.1 hemolysin III family protein -
LN355_RS05275 (1081342) 1081342..1082322 - 981 WP_001564008.1 tRNA-modifying protein YgfZ -
LN355_RS05280 (1082565) 1082565..1082831 + 267 WP_001564007.1 FAD assembly factor SdhE Antitoxin
LN355_RS05285 (1082812) 1082812..1083219 + 408 WP_000244781.1 protein YgfX Toxin
LN355_RS05290 (1083259) 1083259..1083780 - 522 WP_001055874.1 flavodoxin FldB -
LN355_RS05295 (1083892) 1083892..1084788 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LN355_RS05300 (1084813) 1084813..1085523 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LN355_RS05305 (1085529) 1085529..1087262 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T224058 WP_000244781.1 NZ_CP086618:1082812-1083219 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T224058 NZ_CP119580:53230-53382 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10565.15 Da        Isoelectric Point: 5.1599

>AT224058 WP_001564007.1 NZ_CP086618:1082565-1082831 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWMMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT224058 NZ_CP119580:c53180-53118 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9GG32

References