Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 956801..957596 | Replicon | chromosome |
| Accession | NZ_CP086618 | ||
| Organism | Escherichia coli strain RIVM_C039227 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A4Y9BN12 |
| Locus tag | LN355_RS04640 | Protein ID | WP_023909482.1 |
| Coordinates | 956801..957175 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A4Y9BHM8 |
| Locus tag | LN355_RS04645 | Protein ID | WP_023909483.1 |
| Coordinates | 957222..957596 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LN355_RS04610 (952766) | 952766..954031 | - | 1266 | WP_001218869.1 | integrase arm-type DNA-binding domain-containing protein | - |
| LN355_RS04615 (954495) | 954495..954821 | - | 327 | WP_001522265.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LN355_RS04620 (954818) | 954818..955081 | - | 264 | WP_001143297.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| LN355_RS04625 (955153) | 955153..956019 | - | 867 | WP_023909479.1 | DUF4942 domain-containing protein | - |
| LN355_RS04630 (956104) | 956104..956301 | - | 198 | WP_001522268.1 | DUF957 domain-containing protein | - |
| LN355_RS04635 (956313) | 956313..956804 | - | 492 | WP_023909481.1 | DUF5983 family protein | - |
| LN355_RS04640 (956801) | 956801..957175 | - | 375 | WP_023909482.1 | TA system toxin CbtA family protein | Toxin |
| LN355_RS04645 (957222) | 957222..957596 | - | 375 | WP_023909483.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| LN355_RS04650 (957676) | 957676..957897 | - | 222 | WP_023909484.1 | DUF987 domain-containing protein | - |
| LN355_RS04655 (957949) | 957949..958704 | - | 756 | WP_001522280.1 | IS21-like element ISEc10 family helper ATPase IstB | - |
| LN355_RS04660 (958701) | 958701..960200 | - | 1500 | WP_059332257.1 | IS21-like element ISEc10 family transposase | - |
| LN355_RS04665 (960381) | 960381..960857 | - | 477 | WP_001186724.1 | RadC family protein | - |
| LN355_RS04670 (960872) | 960872..961345 | - | 474 | WP_001522284.1 | antirestriction protein | - |
| LN355_RS04675 (961440) | 961440..961616 | - | 177 | WP_001522285.1 | hypothetical protein | - |
| LN355_RS04680 (961616) | 961616..962434 | - | 819 | WP_001522287.1 | DUF932 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX | 765165..960124 | 194959 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14009.98 Da Isoelectric Point: 8.2905
>T224057 WP_023909482.1 NZ_CP086618:c957175-956801 [Escherichia coli]
MKTLSDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRTVNNITQGKHPEAK
MKTLSDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRTVNNITQGKHPEAK
Download Length: 375 bp
>T224057 NZ_CP119579:c46677-46156 [Escherichia coli]
GTGAGCAATACAACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGCCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGCACTCAAGAAG
AAAGGCCAAAAGTGTTAGGATATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCAAGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA
GTGAGCAATACAACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGCCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGCACTCAAGAAG
AAAGGCCAAAAGTGTTAGGATATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCAAGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13598.31 Da Isoelectric Point: 5.5051
>AT224057 WP_023909483.1 NZ_CP086618:c957596-957222 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT224057 NZ_CP119579:c46954-46685 [Escherichia coli]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y9BN12 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y9BHM8 |