Detailed information of TA system TA224054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 206651..207263
• Replicon: chromosome
• Accession: NZ_CP086618
• Organism: Escherichia coli strain RIVM_C039227

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: LN355_RS00940
• Protein ID: WP_000833473.1
• Coordinates: 206651..206836 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: LN355_RS00945
• Protein ID: WP_000499744.1
• Coordinates: 206853..207263 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN355_RS00930 (203492)	203492..204643	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
LN355_RS00935 (204844)	204844..206382	+	1539	WP_001551836.1	aldehyde dehydrogenase AldB	-
LN355_RS00940 (206651)	206651..206836	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
LN355_RS00945 (206853)	206853..207263	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LN355_RS00950 (207335)	207335..209299	-	1965	WP_001551835.1	glycoside hydrolase family 127 protein	-
LN355_RS00955 (209310)	209310..210710	-	1401	WP_000204811.1	MFS transporter	-
LN355_RS00960 (210936)	210936..211751	+	816	WP_000891823.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T224054 WP_000833473.1 NZ_CP086618:206651-206836 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T224054 NZ_CP119577:c4296909-4296736 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT224054 WP_000499744.1 NZ_CP086618:206853-207263 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT224054 NZ_CP119577:c4297233-4296937 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTTATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9