Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 63469..64028 Replicon plasmid pRIVM_C039629_1
Accession NZ_CP086617
Organism Escherichia coli strain RIVM_C039629

Toxin (Protein)


Gene name relE Uniprot ID G4VUF8
Locus tag LN353_RS25265 Protein ID WP_000421273.1
Coordinates 63753..64028 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag LN353_RS25260 Protein ID WP_001387467.1
Coordinates 63469..63753 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN353_RS25235 (58854) 58854..59420 - 567 Protein_73 zinc-binding dehydrogenase -
LN353_RS25240 (59773) 59773..60663 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
LN353_RS25245 (60688) 60688..61068 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
LN353_RS25250 (61101) 61101..62066 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
LN353_RS25255 (62112) 62112..62864 - 753 WP_001403368.1 IclR family transcriptional regulator -
LN353_RS25260 (63469) 63469..63753 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
LN353_RS25265 (63753) 63753..64028 + 276 WP_000421273.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LN353_RS25270 (64134) 64134..64337 + 204 Protein_80 hypothetical protein -
LN353_RS25275 (64462) 64462..64590 - 129 Protein_81 IS481 family transposase -
LN353_RS25280 (64623) 64623..65792 - 1170 WP_001403369.1 hypothetical protein -
LN353_RS25285 (66324) 66324..66592 + 269 Protein_83 type II toxin-antitoxin system ParD family antitoxin -
LN353_RS25290 (66579) 66579..66869 + 291 Protein_84 type II toxin-antitoxin system RelE/ParE family toxin -
LN353_RS25295 (66936) 66936..67118 + 183 WP_042344623.1 hypothetical protein -
LN353_RS25300 (67239) 67239..67979 + 741 WP_001066941.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 - 1..138402 138402


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10765.45 Da        Isoelectric Point: 9.9324

>T224048 WP_000421273.1 NZ_CP086617:63753-64028 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T224048 NZ_CP119577:2687621-2687728 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT224048 WP_001387467.1 NZ_CP086617:63469-63753 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT224048 NZ_CP119577:c2687574-2687508 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6H1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References